Poor development of human nuclear transfer embryos using failed fertilized oocytes
نویسندگان
چکیده
منابع مشابه
the chromosomal abnormality of failed fertilized human oocytes in an in vitro fertilization program
background: the high fertilization failure after ivf treatment cycles could be related to chromosomal abnormalities. this study was carried out to assess the frequency of chromosomal abnormality on human oocytes lacking signs of fertilization 18-20 h after insemination . materials and methods: on day one, 18-20 h after insemination (ivf), fertilization was confirmed when two pronuclei (normal i...
متن کاملComparative stepwise pattern of reactive oxygen species production during in vitro development of fertilized and nuclear transferred goat embryos
Objective A unique feature of embryo metabolism is production of reactive oxygen species (ROS). It is well established that during in vitro culture, ROS levels increase over normal ranges observed for embryos developed in vivo. This study evaluates and compares the stepwise pattern of ROS production during in vitro development of reconstructed goat embryos produced by zona-free method of somati...
متن کاملEffect of activation treatments of recipient oocytes on subsequent development of bovine nuclear transfer embryos.
Effects of recipient oocyte activation methods on the development of nuclear transfer (NT) embryos were investigated. In Exp. 1, cell-cycle phase of serum-starved bovine cumulus cells was examined by flow cytometry. Majority (95.5%) of medium-sized (16-20 microm) cells that made up 56% of total cells was at the G0/G1 phase. NT embryos were constructed by electric fusion with the medium-sized se...
متن کاملEmbryonic development in human oocytes fertilized by split insemination
OBJECTIVE To compare the laboratory outcomes of intracytoplasmic sperm injection (ICSI) and conventional insemination using sibling oocytes in poor prognosis IVF cycles where ICSI is not indicated. METHODS Couples undergoing IVF with following conditions were enrolled: history of more than 3 years of unexplained infertility, history of ≥3 failed intrauterine insemination, leukocytospermia or ...
متن کاملchromosomal abnormalities in failed fertilized oocytes following in vitro fertilization
introduction: the types and incidence of the chromosomal abnormalities in failed fertilized oocytes that were taken after routine in vitro fertilization (ivf) and intracytoplasmic sperm injection (icsi) programs were investingated in patients referred the royan institute. materials and method: in this study, 364 air-dried preparation of failed fertilized oocytes after either ivf (91 samples) or...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2005
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)61693-8